Dynamic Changes and Clinical Significance of White Blood Cells and Platelets in Patients with Traumatic Brain Injury

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He Wang, Kaili Liu, Chao Xiu, Bin Liu, Wantao Zhao, Liang Liu, Jinfeng Pang


Traumatic brain injury (TBI) is becoming the main cause of death threatening human life because of its high disability rate, high mortality rate and great social harm. The purpose of this study was to investigate the dynamic changes and clinical significance of leukocytes (WBC) and platelets (PLT) in patients with traumatic brain injury. In the process of specimen collection, 4ml of peripheral venous blood was collected 24 hours, 4 days, 7 days, 14 days and 21 days after craniocerebral injury using sterile, non-heat source and non-endotoxin test tube. 2ml venous blood was added into EDTA anticoagulant blood collection vessel to detect the changes of peripheral blood leukocytes and platelets in patients with craniocerebral injury. In addition, 2 ml venous blood was added into the procoagulant blood collection vessel, and the serum was separated and frozen for the determination of CRP and TSP1. All patients were evaluated with Glasgow outcome score at discharge. When detecting PLT and WBC, Sysmex K4500 hematology analyzer was used to routinely measure the number of PLT and WBC in peripheral blood at different time points. In the process of CRP (C-reactive protein) measurement, adjust the pipette to 60 μl, and add 50 μl of developer a and developer B into the micropore respectively. Then it vibrates on a mini vibrator for 5 seconds to ensure mixing. Preheat the microplate reader for 30 minutes, place the microplate in the microplate reader, set the parameters and read the values. When measuring TSP1 (thrombin sensitive protein 1), adjust the micropipette to 50 μl, then add 50μl termination solution into the micropore respectively, and then vibrate on the micro vibrator for 30 seconds to ensure full mixing. Further analysis showed that there was no difference between the non-infection group and the normal control group on the 4th day (P > 0.05). This study is helpful to the timely treatment of patients with traumatic brain injury.

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