Effect of Pure and Ethanol Extract of Aloe Vera and Moringa against Streptococcusagalactiae and Staphylococcusaureus Isolated from Mastitis Milk of Buffalo

Abstract

Among medicinal plants, Aloe vera and Moringa are used commonly due to their various therapeutic effects. Hence, this research aimedto see whether there's any antibacterial activityof pure and ethanol extract of Aloe vera and Moringa for some pathogenic organisms isolated from mastitis milk. During the current study, 50 mastitis milk samples were obtained from different dairy farms of the nearby Tandojam under aseptic conditions and brought to the Department of Veterinary Pharmacology for microbial culture analysis. After isolation and identification of the organisms, the disc diffusion method did the antibacterial activity. For this purpose, 30µg/µl and 60 µg/µl of Aloe vera and Moringa and their ethanol extracts were used against Staphylococcus aureus (S. aureus) and Streptococcus agalactiae (Strep. agalactiae).Results revealed that out of 50 mastitis milk samples analyzed, 25 (50%) were found positive for S. aureus,and 20(40%) were positive for Strep. agalactiae and 5 (10%) were detected positive for mixed colonies. The mean growth inhibition of S. aureus against 30 µg/µl of pure Aloe vera was recorded(2.83mm), and for Strep. Agalactiae (2.36 mm) and on 60µg/µl, both organisms reacted more sensitive and exhibited inhibitory zone of (8.03mm) and (7.1mm), respectively. At 30µg/µl, ethanol extract of Aloe vera yielded (7.9mm) sensitivity for S. aureus and Strep. agalactiae (6.46mm), and on 60µg/µl, the isolated organisms remained highly sensitive and showed an inhibitory zone of (16.66mm) for S. aureus and (13.26mm)for Strep. agalactiae. However. Pure Moringaat 30µg/µl, inhibition zone for S. aureus recorded was (2.37mm) and Strep. agalactiae showed (2.36 mm) sensitivity while, on 60µg/µl, the organisms responded more sensitive and presented an inhibitory zone of (8.03mm) and (7.1mm), respectively. Though, 30µg/µl of ethanol extract of Moringa showed (7.9mm) sensitivity on S. aureus and Strep. agalactiae showed (6.46mm) sensitivity, but on 60µg/µl of ethanol extract, it persisted as highly sensitive against isolated organisms and produced an inhibitory zone of (14.43mm) for S. aureus and(12.56mm) for Strep. agalactiae. Thus, it has been concluded that all treatments hampered the growth of isolated organisms, but pure Aloe vera and its ethanol extract exhibited better inhibition zones than Moringa.