Assessment of Leptin, TNF-α and Visfatin Markers in Type 1 Diabetic Children

Abstract

Objectives:The main objective of this research is to provide statistical predictive model(s) to identify patients with high-risk ""type 1 diabetes mellitus"". Furthermore, we aimed to evaluate visfatin, leptin, and TNF-α in children with T1DM regarding glycemic state and their BMI and use these statistical analytics solutions with the logistic regression predictive model results to check the diagnostic potential for these markers in the future treatment of T1DM.

Methods: The study included a hundred diabetic patients, which were classified based on HbA1c level into controlled and uncontrolled groups. They were also categorized according to their BMI: normal weight, overweight, and obese. ELISA and real-time PCR were used for detection.

Results: There was an elevation in leptin and TNF-α serum, while C-peptide and visfatin decreased significantly in patients.The uncontrolled diabetics showed higher leptin and TNF-α but lower C-peptide and visfatin than controlled diabetics. While increasing leptin gene expression and TNF-α levels in uncontrolled diabetic group but visfatin decreases. 

Conclusion: The uncontrolled diabetic group was associated with higher weight, BMI, and higher leptin gene expression than controlled diabetics. We found a significant impact of both markers on the prediction of T1DM with high sensitivity and specificity and even with the acceptable significance of p-values.