Investigating the Impact of Methanol and Salicylic Acid Foliar Application under Deficit Irrigation Conditions on Enzymatic Traits of Brasicanapus L.

Abstract

This study was conducted to investigate the impact of foliar application of salicylic acid and methanol on enzymatic traits of Brasicanapus L. Hyola 401 under the field experimental drought stress as a factorial split plot in a randomized complete block design with three replicates during the two crop years of 2017 and 2018 in Safi Abad agricultural and natural resources research and education center of Dezful. In order to do this experiment in the main plot, drought stress treatment including three levels (70, 140 and 210mm evaporation) and in the sub-plot salicylic acid foliar application treatments in three levels (control, 100 and 20 μmol) and Methanol were administered at three levels (control, 10 and 20 volume percent). The results of variance analysis showed that the effect of drought stress on the activity of enzymes of catalase, guaiacol peroxidase and ascorbate peroxidase and also, on the amount of soluble carbohydrates and proline was significant at 1% probability level (P <0.01). Foliar application of salicylic acid and methanol was also significant on the measured traits at 1% probability level (P <0.01). The results of the mean comparison indicate the existence of interactions between the three factors studied in this experiment in the traits of catalase, guaiacol peroxidase and proline. The overall obtained results of this study showed that drought stress increased the activity of antioxidant enzymes as well as the soluble carbohydrates content and proline.