Effect of Pomegrante Extract (Punica Granatum( on Interleukin Levels and Liver Enzyme in Mice

Abstract

   Pomegranate (Punica granatum) crude plant extract has been prepared by way of using ethanol 80% solvent. Dried peeling and pulp in room temperature, 100 gm weight of powder plant and was solubilized in 500 ml of 80% ethanol alcohol. The consequences of phytochemical screening of crude extracts found that: alkaloids, flavonoids, tannins, phenolic and glycosides had been present in ethanolic extract while resins, saponines and violet oils had been absent. In vivo observe discovered that, the effect immunized mice with a 100 μl (50 mg/kg) of ethanol crude extracts, were given subcutaneously and orally for 14 days on extraordinary parameters. The first its effect on immune markers in mice (Interleukins 4, 6 and TNF). The second its effect on biochemical exams for liver enzymes (GOT and GPT) as compared to control mice were intraperitoneal injected with LPS alone (1 mg/kg body weight), damage of liver by LPS was induced. Than pre-treated mice with (50 mg/kg) extracts subcutaneously and orally for 14 days injected with the LPS on day 15 (after 14 days administration  of extracts). The results showed that there was an decrease in the (IL-4), (IL-6) and (TNF) compared to the control mice group, cause decreases the immune system when use pomegranate crude extract contains bioactive compounds present a wide range of flavonoids, polyphenolic,  and other anti-inflammatory phytochemicals compared with control. When mice were exposed to extracts with LPS, GOT and GPT levels decrease in all groups compared to injected control LPS group. The protective effect of extracts on hepatic damage, induced by LPS, was investigated.